Study Design
I. Serum collection for "Pretransplant Serum Study":
- One pretransplant serum, preferably last serum before transplantation
II. Serum collection for "Posttransplant Serum Study":
- Pretransplant serum, preferably last serum before transplantation
- 2 sera during the first posttransplant week, preferably on days 2 and 6
- 1 serum at the end of the 1st posttransplant month
- Not obligatory - but if possible - additional sera at the end of month 3, 6, 12, and 24, obtained during routine clinical visits
- Not obligatory - but if possible - recipient and donor DNA
| Serum Collection Scheme | |||
| Pre-Tx | First Post-Tx Week | One Month | Additional Sera |
| Day 0 | Days 2, 6 | Day 30 | approx. Days 90, 180, 365, 730, yearly thereafter |
Serum samples:
1-2 ml serum without additives (minimum 0.5 ml). Two hours after blood donation, the serum should be separated from cell material by centrifugation and frozen and stored at -20°C.
DNA samples:
EDTA blood, buffy coat and in the case of donor also spleen tissue can be frozen and stored at -20°C
Shipment of frozen samples to Heidelberg, Germany, at dates to be announced (twice a year).
Documentation:
- Regular CTS questionnaires (no additional paper work)
-
One additional 3-month questionnaire: (Download study form)
- Graft function within 24 hours: Yes / No
-
Rejection during first 3 months: Yes / No
- If yes: Date first rejection diagnosed: ... (Day/Month/Year)
- If yes: Banff-97 Grading (if available): ...
-
Acute Tubular Necrosis (ATN): Yes / No
- If yes: Was patient dialyzed? Yes / No
- Calcineurin inhibitor-induced nephrotoxicity: Yes / No
- Infection during the first week: Yes (viral, bacterial, fungal) / No